Methods for Fragments Screening Using Surface Plasmon Resonance

使用表面等离子体共振筛选片段的方法

生物化学

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出  版 社
出版时间
2021年09月26日
装      帧
精装
ISBN
9789811615351
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语      种
英文
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图书简介
This volume describes mThods and prTocols forThe fragmeT-based screening of prTeins using Surface Plasmon Resonance (SPR).The inTial chaTer ofThe book discussesThe principle of SPR forThe ideTificTion of biomolecular iTeraTions, whileThe subsequeT chaTers iTroduce mThods for labelling prTeins wTh differeTTags including, hiTidine and biTinTags. T also discussesTechniques and faTorsThT affeTThe amine and biTin-TreTavidin coupling and mThodsTo oTimizeThe iTeraTions. NeT, T describes fragmeT preparTion for screening in SPR and preseTs mThodsTo calculTe equilibrium dissociTion conTaT (KD) and ligand efficiency (LE). T reviewsTechniques of neT-generTion injeTionsThT improveThe efficiency ofThe charaTerizTion process overTradTional SPR by dTerminingThe kinTics and affinTy in a single Tep. Finally,The book elucidTes a comprehensive yT represeTTive descriTion of challenges associTed wThThe molecular iTeraTion of prTeins using SPR.
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