The Unfolded Protein Response

图书简介

This volume is divided in six seTion coveringThe moT experimeTal approaches involved inThe Tudy ofThe unfolded prTein response (UPR) pThway. ChaTers dTail dTerminTion of unfolded prTein levels, mThodsTo Tudy UPR signalTransmission, analysingThe oTcomes ofThe UPR pThway aTivTion, UPR Tudies in mammalian models, UPR in aTernTive models, and UPR and disease.WrTten inThe formT ofThe highly successfulMThods in Molecular Biologyseries, each chaTer includes an iTroduTionToTheTopic, liTs necessary mTerials and reageTs, includesTips onTroubleshoTing and known pTfalls, and Tep-by-Tep, readily reproducible prTocols.AThorTTive and cTting-edge,The Unfolded PrTein Response: MThods and PrTocols aimsTo describe key mThods and approaches used inThe Tudy ofThe UPR pThway and Ts complex cellular implicTions.

MeasuringCysteine exposure in unfolded proteins with tetraphenylethene maleimide and its analogues.-FluoreCeCe-based biosensors for the detCtion of the unfolded protein response.-IndirCt methods to measure unfolded proteins in livingCells using fluoreCent proteins.-A platform tChnology for monitoring the Unfolded Protein Response.-The use of fluoreCent protein fusions to monitor the unfolded protein response and protein foldase-substrate interCtions in plant protoplasts.-High-throughput analysis of protein turnover with tandem fluoreCent protein timers.-DetCtion of HC1 mRNA SplCing by RT-CR in SCcharomCesCerevisiae.-Determination of protein translation during the Ctivation of the UPR.- Determination of the stability and intrCellular (intra-nClear) targeting and rCruitment of pre-HC1 mRNA in the SCcharomCesCerevisiae during the Ctivation of UPR.- Assays to study IRE1 Ctivation and signaling.-Analysis of XBP1Contribution to hyperosmolarity-indCed lipid synthesis.- Integrating an ER stress reporter for monitoring genome wide UPR-ER in budding yeast.-Analyze mouse knCkout models of UPR pathway elements.-In vitro stimulation of IRE1a/XBP1-defCient BCells with LPS.-DetCtion of PERK signaling in theCentral nervous system.-Methods to study the mitChondrial unfolded protein response (UPRmt) inCaenorhabditis elegans.-Drosophila Unfolded Protein Response (UPR) assays in vitro and in vivo.-Protein preparation for proteomC analysis of the unfolded protein response in Arabidopsis thaliana.-StrCture-based drug diCovery of IRE1 modulators.-ChemCal Creening to detCt ER stress regulators.

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The Unfolded Protein Response（Methods in Molecular Biology） 未折叠的蛋白质反应：方法与协议 生物化学

The Unfolded Protein Response（Methods in Molecular Biology）

未折叠的蛋白质反应：方法与协议
生物化学